2011•Nanjing Zhongyiyao Daxue xuebaoRequires access
Determination of Solvent Residues in Polysorbate-80 by Headspace Gas Chromatography
Jun Li
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Abstract
OBJECTIVE To determine the solvent residues in polysorbate-80 by establishing headspace gas chromatography(HS-GC).METHODS HS-GC with capillary column Agilent DB-624 as chromatographic column and n-butanol as the internal standard was adopted.RESULTS The linear ranges of chloroform,dichloromethane,ethylene glycol and diethylene glycol were 6.73～1 346 μg/mL,6.34～1 268 μg/mL,3.30～660 μg/mL and 1.87～374 μg/mL respectively.The detection limits of the four residual solvents were 2.02,1.90,0.009 9 and 0.005 7 μg/mL respectively.CONCLUSION This method is proved to be sensitive,accurate and specific.It can effectively determine the four solvent residues in polysorbate-80.