Detection of ESBLs produced by Escherichia coli and Klebsiella pneumonia and drug resistance monitoring

Objective: To investigate the status of extended-spectrum B-lactamases(ESBLs) production by Escherichia coli and Klebsiella pneumonia,analyze the characteristics of drug resistance of Escherichia coli and Klebsiella pneumonia in our hospital from Jan 2003 to June 2008,in order to provide a guidance to clinical rational use of antibiotics.Methods:The strains were identified by API microbiological assay system.ESBLs-producing Disc diffusion test(K-B method)was used to study the drug resistance of E.coli and K.pneumonia isolates,and ESBLs-producing strains were identified with screening test and confirmatory test.WHONET5.3software was apllied to performing data analysis.Results:In 727strains of E.coli,162 strains(22.3%) were detected to produce ESBLs;in 465strains of K.pneumonia,122 strains(26.2%) were detected to produce ESBLs.The resistance rate of ESBLs-producing strains was significantly higher than that of ESBLs nonproducing ones(P0.05),and but ESBLs-producing strains displayed multi-resistant.Conclusion:Monitoring of ESBLs producing strains and the drug-resistance can contribute to guide the clinically reasonable use of antibiotics.