Detection of ESBLs produced by Escherichia coli and Klebsiella pheumoniae and drug resistance monitoring

Objective To investigate the status of extended-spectrum β-lactamases(ESBLs)production by Escherichia coli and Klebsiella pheumoniae,analyze the characteristics of drug resistance of Escherichia coli and Klebsiella pheumoniae in our hospital from Jan 1998 to Dec 2006,in order to provide a guidance to clinical rational use of antibiotics.Methods Disc diffusion test(K-B method)was used to study the drug resistance of E.coli and K.pheumoniae isolates,and ESBLs-producing strains were identified with screening test and confirmatory test.WHONET5.4 software was apllied to performing data analysis.Results In 947 strains of E.coli,311 strains(32.8%)were detected to produce ESBLs;in 293 strains of K.pheumoniae,69 ESBLs-producing strains(23.5%)were detected.The resistance rate of ESBLs-producing strains was significantly higher than that of ESBLs non-producing ones(P0.05),and but ESBLs-producing strains displayed multi-resistant.Conclusion The positive rate of ESBLs producing strains is higher in our hospital.Drug resistance monitoring should be strenghed to rationally use antibacterials and to prevent increase,prevalence and outbreak of ESBLs producing strains.