2005•Zhonghua shiyan waike zazhiRequires access
Effects of enteral immunonutrition on endotoxin and the expression of CD14/TNF-αmRNA in rats after thermal injury
Guanghua Guo
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Abstract
Objective To investigate the effects of enteral immunonutrition on endotoxin and the expression of CD14/TNF-α mRNA in severe scalded rats. Methods Sixty-four SD rats inflicted by 30% TBSA III degree scalds were randomly divided into enteral immunonutrition (EIN, n = 32) group and enteral nutrition (EN, n = 32) group. The animals in two groups were fed with isocaloric enteral nutrition. The serum levels of endotoxin and TNF-α were determined at first, 4th, 7th and 10th day after injury. Simultaneously, the liver tissues were collected for the detection of the CD14 mRNA and TNF-α mRNA expression. Results Compared with pre-injury, the serum levels of endotoxin and TNF-α were significantly increased and the expression of CD14 mRNA and TNF-α mRNA in liver tissues also increased obviously in both groups. However the serum levels of endotoxin and the expression of CD14 mRNA, TNF-α mRNA in liver tissues in EIN group were significantly lower than those in EN group on the day 4, 7 and 10 post-injury (P0.05 or P0.01).Conclusion Enteral immunonutrition superior to enteral nutrition in the early stage of sever scalded injury could decrease the serum levels of endotoxin and TNF-α, reduce the expression of CD14 mRNA and TNF-α mRNA in liver tissues and improve inflammatory responses.