Effect of growth hormone on inflammatory response of liver after cardiopulmonary bypass in rats

Objective To study the effects and mechanisms of growth hormone(GH) pretreatment on the inflammatory response of liver in rats after cardiopulmonary bypass(CPB).Methods The CPB model in rat was established.The rats were randomly divided into GH pretreatment group(GH group),CPB group,and sham group.The serum levels of endotoxin,TNF-α,and liver function were determined at different intervals respectively.Simultaneously,the liver tissues were collected for the detection of the CD14 mRNA and TNF-α mRNA by RT-PCR.Results Compared with pre-CPB,the serum levels of endotoxin,TNF-α were significantly increased,and the lesion of liver function was apparent in GH group and CPB group.Meanwhile,the expression of CD14 mRNA and TNF-α mRNA in liver tissues were also increased obviously in both groups.However,the serum levels of endotoxin and TNF-α and the expression of CD14 mRNA and TNF-α mRNA in liver tissues at the same time point in GH group were significantly lower than those in CPB group(P0.05 or P0.01).Compared with CPB group,the liver function at the same time point improved in GH group.Conclusion GH pretreatment could decrease the serum levels of endotoxin and TNF-α,reduce the expression of CD14 mRNA and TNF-α mRNA in liver tissues,relieve inflammatory response and improve the liver function.