Hongshan Yu
Abstract
Gene rutin-α-rhamnosidase was subcloned with shuttle plasmid pPIC9K and expressed in Pichia pastoris strain GS115.The expression of rutin-α-rhamnosidase was detected after incubation for 144 hours when methanol was used as carbon source.The target protein was 53 ku in molecular weight by detected via SDS-PAGE.
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Gene rutin-α-rhamnosidase was subcloned with shuttle plasmid pPIC9K and expressed in Pichia pastoris strain GS115.The expression of rutin-α-rhamnosidase was detected after incubation for 144 hours when methanol was used as carbon source.The target protein was 53 ku in molecular weight by detected via SDS-PAGE.
Key concepts: Pichia pastoris, Rutin, Chemistry, Incubation, Plasmid, Recombinant DNA, Molecular biology, Expression vector