Sub-cloning and expression of ginsenoside-α-rhanmosidase gene

Gene ginsenoside-α-rhamnosidase was subcloned into an expression vector pPIC9K,the Pichia pastoris GS115 cells were transformed with the recombinant plasmid by electroporation and expressed.After induction for 72 hours and adding 0.5% methanol,it was showed that the target protein was expressed in Pichia pastoris GS115 by analyses of the TLC and SDS-PAGE.