2003•Unpublished venueRequires access
Expression of β-glucosidase in Pichia pastoris expression system
Li Zeng
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Abstract
One βglucosidase gene in Arabidopsis thaliana (AT5g44640) was expressed using Pichia pastoris expression system and the active target protein was obtained. The factors affecting the expression of Pichia were also analyzed. The results showed that copy number of insertion of the expression cassette, strains of P.pastoris, different methanol concentrations, time of induction and beginning concentration of induction all had influence on the yield of βglucosidase.