Effects of Angiotensin(1-7) on the Expression of Lectin-Like Oxidized Low Density Lipoprotein Receptor-1 in Human Umbilical Vein Endothelial Cells Induced by Angiotensin II

Aim To investigate the effects of Angiotensin(1-7)(Ang(1-7)) on the expression of lectin-like oxidized low density lipoprotein receptor-1(LOX-1) in human umbilical vein endothelial cells(HUVEC) induced by angiotensinⅡ(AngⅡ).Methods HUVEC were isolated and cultured.Cultured HUVEC were incubated for 24 h with Ang-(1-7),AngⅡ,Ang(1-7)+A-779,Ang(1-7)+AngⅡ,A-779+AngⅡ+Ang(1-7),respectively.And cultured HUVEC without incubating stimulator were chosen as controls.The expression of the mRNA and protein of LOX- 1 was measured by RT-PCR and flow cytometry methods respectively.The expression of the phosphorylation of p38 mitogen activated protein kinase(MAPK) was determined by Western blotting.Results Compared with the AngⅡgroup, Ang(1-7) dose-dependently inhibited the expression of LOX-1 in HUVEC(P0.05).Compared with control group, the expression of the phosphorylation of p38 MAPK increased significantly in AngⅡgroup(P0.05),while it was a little in Ang(1-7) group and A-779 group(P0.05).The expression of the phosphorylation of p38 MAPK reduced in AngⅡ+Ang(1-7)group compared with AngⅡgroup(P0.01).The expression of the phosphorylation of p38 MAPK had no significant change in AngⅡ+Ang(1-7)+A-779 group(P0.05).Conclusion Ang(1-7) concentration dependently attenuate the expression of LOX-1 induced by AngⅡin HUVEC through its specfic receptor MAS,Ang(1-7) could inhibit the expression of the phosphorylation of p38 MAPK induced by AngⅡin HUVEC,and then inhibit the AngⅡ-induced LOX-1 espression.