Effect of angiotensin II on phosphorylation of p38 Mitogen-activated protein kinases in HUVECs

Objective To investigate the expression of the phosphorylation of p38 Mitogen-activated protein kinases (p38MAPK) induced by angiotensin Ⅱ (Ang Ⅱ) in human umbilical vein endothelial ceils (HUVECs). Methods Cultured HUVECs were separatedly incubated for 16h with non-stimulators:different time point (0,5,10, 15,30,45,60min) of the observation group with Ang Ⅱ of 100 nmol/L; different dose of Ang Ⅱ(0,10,100,1000,10 000 nmol/L) with a fixed stimulation time of 15 min; Ang Ⅱ + the specific inhibitors of Ang Ⅱ,SB202190.The expression of the phosphorylation of p38MAPK was determined by Western blot.Results Following excitation by Ang Ⅱ(100 nmol/ L),there was substantial up-regulation of p38MAPK phosphorylation in HUVECs with maximal activation at 15-30 min (2.25±0.21,2.51±0.16,P0.005); Ang Ⅱ increased the expression of the phosphorylation of p38MAPK in a dosedependent manner; Pre-treatment with SB202190 (1000,5000 nmol/L) for 30 min before Ang Ⅱ (100 nmol/L)was used, the expression of phosphorylation of p38MAPK was inhibited markedly in a dose-dependent manner.The inhibition rate was 53.9% (P0.01) in SB202190 (5000 nmol/L) group.Conclusions Ang Ⅱ can activate the expression of the phosphorylation of p38MAPK in HUVECs,then induce the inflammation.