Influence of angiotensin-(1-7) on the secretion of MCP-1 and ICAM-1 in cultured human umbilical vein cells induced by angiotensin II

AIM:To explore the influence of angiotensin-(1-7)[Ang-(1-7)]on the secretion of monocyte chemoattractant protein (MCP-1) and intercellular adhesion molecule-1 (ICAM-1) in human umbilical vein endothelial cells (HUVEC) induced by angiotensin II (AngⅡ),and to clarify the prevent effect of Ang-(1-7) on the inflammation induced by AngⅡ.METHODS:The cultured HUVEC were randomly divided into control group,AngⅡ group,Ang-(1-7) group,Ang-(1-7) + AngⅡ group,and A-779 + Ang-(1-7) + AngⅡ group. The expressions of MCP-1 and ICAM-1 in protein level and mRNA level were detected by enzyme linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR).RESULTS:Compared with the control group,100 nmol/L AngⅡ distinctly increased the protein and mRNA expression of MCP-1 and ICAM-1 in HUVEC (P0.05). Ang-(1-7)(1 000 nmol/L) decreased the protein and mRNA expression of MCP-1 and ICAM-1 (P0.05). Compared with the AngⅡ group,Ang-(1-7) inhibited the protein and mRNA expressions of MCP-1 and ICAM-1 in HUVEC induced by AngⅡ in a dose-dependent manner (P0.05). When the concentration of Ang-(1-7) was up to 1 000 nmol/L,the expressions of ICAM-1 and VCAM-1 were more than that in control group,but was not statistically significant (P0.05). No significant difference between the A-779 group and AngⅡ group was observed (P0.05).CONCLUSION:Ang-(1-7) inhibits the secretion of MCP-1 and ICAM-1 in cultured HUVEC in a concentration dependent matter induced by AngⅡ. The inhibitory effect of Ang-(1-7) may be through its specific receptor.