Laboratory Tests of SLE Patients with Negative ANA

Objective To determine the diagnosis value and clinical significance of autoantibody and humoralimmunity in SLE patients with negative ANA.Methods ANA was tested by indirect immunofluoresence technique;Anti-dsDNA antibody was examined by erithidia lueiliae immunofluresent technique;Extractable nuclear antigen(ENA) was detected by immunobloting technique;immunoglobins and complements were tested by rate nephelometry technique.Results Compared with other auto immune diseases(AID),the positive rates existed significant difference among anti-dsDNA antibody,anti-SM antibody and anti-nucleosome antibody in SLE patients with negative ANA.while the differences of positive rates did not exist between the two groups among IgG,IgA,IgM,C3 and C4.Conclusion Positive results of anti-dsDNA antibody,anti-SM antibody and anti-nucleosome antibody have great value to SLE patients with negative ANA,while immunoglobins and complements have reference value.