The effect of bone marrow stem cells mobilization on cardiac performance of acute myocardial infarction rats

AIM: To explore the effect and the possible mechanism of G-CSF and SCF on cardiac function by mobilizing the bone marrow stem cells in acute myocardial infarction rats. METHODS: Myocardial infarction of left ventricle was induced in male Wistar rats by ligation of left coronary artery. The rats(n40)were randomly divided into 2 groups: ①The mobilized group receiving hypoderm injection of rhG-CSF and rh-SCF, and ② The control group receiving hypoderm injection of an equivalent amount of normal saline. After 28 days, heart function was examined and hearts were harvested for HE stain,immunofluorescence and double immunofluorescence. Myocardial ischemic size and infarct size were determined by dual staining with Evan's blue dye and triphenylterazolium chloride. RESULTS: Compared with the control group, fibrosis was less in the infarct zone of the mobilized group. Regenerative vasculars and myocytes specific proteins were stained positively. Left ventricular systolic pressure,left ventricular end-diastolic pressure and left ventricular pressure decay ±dp/dt_(max) of the mobilized group were higher than those of the contral group (P0.05).There was statistical differences between the two groups. Myocardial ischemic size and infarct size of the mobilized group[(41±7)% and (43±6)%] were statistically smaller than those of contral group;[(53±8)% and (55±10)%](P0.05). CONCLUSION: By using G-CSF and SCF , the heart function of acute myocardial ischemic rats is improved.