Mononuclear bone marrow cells transplanting in treatment of myocardial infarction

Objective:To investigate the therapeutic effect of mononuclear bone marrow cells(MBMCs) transplanting in treatment of myocardial infarction. Methods:Under sterile environment, the femur and the tibia from adult male Wistar rats(inbreeding line) were obtained, bone marrow was extracted with routine method, and MBMCs were separated and made into suspension. Forty-six adult Wistar rats were divided into 3 groups at random: blank (group Ⅰ,n=10),control (groupⅡ,n=18) and transplantion (group Ⅲ,n=18). Myocardial infarction was induced by ligation of left anterior descending coronary artery. GroupⅡwere injected with 10 μl phosphate buffered saline(PBS) and group Ⅲ were transplanted with MBMCs (6×10 6)/10 μl 2 weeks after myocardial infarction. Four weeks after MBMCs transplantation, echocardiography and hemodynamics examination were performed in all groups to observe the date of ejection fraction(EF),fractional shortening(FS) and left ventricular anterior wall thickness(LVAWT) for evaluation of the cardiac function and left ventricular remodeling. The changes of left ventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP) and the maximal ratio of left ventricular diastolic pressure to left ventricular diastolic time(±dp/dt max ) were also measured for evaluation of hemodynamic changes.The survival,differentiation of transplanted MBMCs and angiogenesis were observed by immunohistochemistry,confocal laser scanning micrography and transmission electron micrography.Results:Four weeks after MBMCs transplantation,the curative effects on cardiac function were obvious. The date of EF,FS and LVAWT were improved compared with those of control group(P0.05) and so were the hemodynamics data,including LVSP,LVEDP and dp/dt max (P0.05).Six weeks after MBMCs transplantation, the eximinations showed that the transplanted cells survived and proliferated. The hearts with transplanted cells had better cardiac function than the control hearts did(P0.05). Conclusion: Transplantation of MBMCs can improve cardiac function obviously in cardiac infarction, which may be attributed to MBMCs-associated myocardial regeneration and neovascularization.