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2011•Journal of Hubei University of Chinese MedicineRequires access

Determination of Chlorogenic Acid in Yinqiao Jiedu Granules by RP-HPLC

Xiang Meixian

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Abstract

Objective To establish the method for determining Chlorogenic acid in Yinqiao Jiedu granules by RP-HPLC.Methods The HPLC conditions with DIONEX-ACCLAIM.120 C18 column(250mm×4.6mm,5μm)was used,the mobile phase was composed of methy1 cyanide and and PBS(pH=2.5) with gradient elution;flow rate was 1.0mL/min;column temperature was 35℃;the detective wavelength was 350nm.Results The linear ranges of Chlorogenic acid was 0.024μg-0.384μg/mL;r=0.9999.The average recoveries(n=6) of Chlorogenic acid was 93.56%;RSD was 1.15%.Conclusion The method is accurate,reproducible and highly selective,thus suitable for quality control of Yinqiao Jiedu granules.

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What this paper is about

Objective To establish the method for determining Chlorogenic acid in Yinqiao Jiedu granules by RP-HPLC.Methods The HPLC conditions with DIONEX-ACCLAIM.120 C18 column(250mm×4.6mm,5μm)was used,the mobile phase was composed of methy1 cyanide and and PBS(pH=2.5) with gradient elution;flow rate was 1.0mL/min;column temperature was 35℃;the detective wavelength was 350nm.Results The linear ranges of Chlorogenic acid was 0.024μg-0.384μg/mL;r=0.9999.The average recoveries(n=6) of Chlorogenic acid was 93.56%;RSD was 1.15%.Conclusion The method is accurate,reproducible and highly selective,thus suitable for quality control of Yinqiao Jiedu granules.

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Available abstract

Objective To establish the method for determining Chlorogenic acid in Yinqiao Jiedu granules by RP-HPLC.Methods The HPLC conditions with DIONEX-ACCLAIM.120 C18 column(250mm×4.6mm,5μm)was used,the mobile phase was composed of methy1 cyanide and and PBS(pH=2.5) with gradient elution;flow rate was 1.0mL/min;column temperature was 35℃;the detective wavelength was 350nm.Results The linear ranges of Chlorogenic acid was 0.024μg-0.384μg/mL;r=0.9999.The average recoveries(n=6) of Chlorogenic acid was 93.56%;RSD was 1.15%.Conclusion The method is accurate,reproducible and highly selective,thus suitable for quality control of Yinqiao Jiedu granules.

Key concepts: Chlorogenic acid, Chromatography, Chemistry, High-performance liquid chromatography, Gradient elution, Elution

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