Effect of Digupi Ethanol Extract on the Renal Expression of Cox-2 in Type 2 Diabetic Rats

Objective:To observe the effect of Digupi extract on renal expression of cox-2 in rats with type 2 diabetic mellitus(DM),and to reveal the underlying mechanism.Methods:Sprague Dawley rats were given high fat diet for 3 weeks to establish type 2 DM obesity.Obese rats were randomly divided into model group,rosiglitazone group,Digupi ethanol extract low dose group(low Digupi group),and Digupi ethanol extract high dose group(high Digupi group),10 in each.Other 10 rats served as normal control.The model and normal control groups were given saline and other groups were intragastrically given respective drugs for 7 weeks.Then rats were sacrificed to detect the expression of cox-2 in the kidney by immunohistochemistry.Results:The expression of cox-2 was significantly reduced in the rosiglitazone group and Digupi groups as compared with that in the model group(P0.01),but it was still higher than that in the normal control group(P0.01).No significant difference was noted between the rosiglitazone group and the high Digupi group(P0.05).Conclusion:Digupi ethanol extract can reduce the renal expression of cox-2 in type 2 diabetic rats.High dose of Digupi had a better effect,which was similar to the efficacy of rosiglitazone.