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Determination of Berberine Hydrochloride in Zuning Granules by HPLC

Wang Ping-tao

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Abstract

Objective To establish a high performance liquid chromatography(HPLC) method for determination of berberine hydrochloride in zuning granules.Methods The HPLC method was employed to determine berberine hydrochloride.The separation was achieved on a Dionex C18 column(4.6 mm × 250 mm,5 μm) at a flow rate of 1.0 mL·min-1.The mobile phase is acetonitrile-0.1% H3PO4(50:50).The detection wavelength was 265 nm,and the column temperature was set at room temperature.Results The linear range of berberine hydrochloride was within 5-50 μg(r=0.999 9).The average recovery was 100.201% and RSD was 0.49%.Conclusion The method is simple,accurate and repeatable.It is proved to be suitable for the quality control of zuning granules.

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What this paper is about

Objective To establish a high performance liquid chromatography(HPLC) method for determination of berberine hydrochloride in zuning granules.Methods The HPLC method was employed to determine berberine hydrochloride.The separation was achieved on a Dionex C18 column(4.6 mm × 250 mm,5 μm) at a flow rate of 1.0 mL·min-1.The mobile phase is acetonitrile-0.1% H3PO4(50:50).The detection wavelength was 265 nm,and the column temperature was set at room temperature.Results The linear range of berberine hydrochloride was within 5-50 μg(r=0.999 9).The average recovery was 100.201% and RSD was 0.49%.Conclusion The method is simple,accurate and repeatable.It is proved to be suitable for the quality control of zuning granules.

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Available abstract

Objective To establish a high performance liquid chromatography(HPLC) method for determination of berberine hydrochloride in zuning granules.Methods The HPLC method was employed to determine berberine hydrochloride.The separation was achieved on a Dionex C18 column(4.6 mm × 250 mm,5 μm) at a flow rate of 1.0 mL·min-1.The mobile phase is acetonitrile-0.1% H3PO4(50:50).The detection wavelength was 265 nm,and the column temperature was set at room temperature.Results The linear range of berberine hydrochloride was within 5-50 μg(r=0.999 9).The average recovery was 100.201% and RSD was 0.49%.Conclusion The method is simple,accurate and repeatable.It is proved to be suitable for the quality control of zuning granules.

Key concepts: Berberine hydrochloride, Chromatography, High-performance liquid chromatography, Chemistry, Berberine, Acetonitrile, Content determination, Linear range

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