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2011•Modern Chinese MedicineRequires access

Determination of Kansuiphorin D in Kansui Radix and its Processed Products

Lin Rui-chao

Open publisher page 1 citations

Abstract

Objective:To develop an HPLC method for determination of Kansuiphorin D in Kansui Radix and to compare the differences of the contents of euphol and tirucallol in Kansui Radix and its processed product (processed with vinegar) by HPLC.Methods:The sample was extracted by methanol.HPLC analysis was performed on an lunar C18 column,column temperature was 40℃.The mobile phase was the system of acetonitrile-water(70∶30).The flow rate was 1.0 mL·min-1 and UV detection was set at 235 nm.Results:Determined the contents of Kansuiphorin D of 44 samples by HPLC.Conclusion:The methad was simple,rapid and accurate with good productivity,which can be used to determine of Kansuiphorin D in Kansui Radix and its processed product.

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What this paper is about

Objective:To develop an HPLC method for determination of Kansuiphorin D in Kansui Radix and to compare the differences of the contents of euphol and tirucallol in Kansui Radix and its processed product (processed with vinegar) by HPLC.Methods:The sample was extracted by methanol.HPLC analysis was performed on an lunar C18 column,column temperature was 40℃.The mobile phase was the system of acetonitrile-water(70∶30).The flow rate was 1.0 mL·min-1 and UV detection was set at 235 nm.Results:Determined the contents of Kansuiphorin D of 44 samples by HPLC.Conclusion:The methad was simple,rapid and accurate with good productivity,which can be used to determine of Kansuiphorin D in Kansui Radix and its processed product.

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Available abstract

Objective:To develop an HPLC method for determination of Kansuiphorin D in Kansui Radix and to compare the differences of the contents of euphol and tirucallol in Kansui Radix and its processed product (processed with vinegar) by HPLC.Methods:The sample was extracted by methanol.HPLC analysis was performed on an lunar C18 column,column temperature was 40℃.The mobile phase was the system of acetonitrile-water(70∶30).The flow rate was 1.0 mL·min-1 and UV detection was set at 235 nm.Results:Determined the contents of Kansuiphorin D of 44 samples by HPLC.Conclusion:The methad was simple,rapid and accurate with good productivity,which can be used to determine of Kansuiphorin D in Kansui Radix and its processed product.

Key concepts: Radix (gastropod), Chromatography, High-performance liquid chromatography, Chemistry, Biology, Botany

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