Effects of G-CSF in Treatment of Acute Myocardial Infarction in Rats

Objective:To investigate the effects of granulocyte colony-stimulating factor(G-CSF) mobilizing bone stem cells in treatment of acute myocardial infarction(AMI) in rats.Methods:32 SD masculine rats were randomly divided into 2 groups(every group possess 16 rats) after injected isoproterenol(ISO) and created AMI model.A group:G-CSF group,B group:control group.Every group was randomly divided into 2 subgroups.Hearts of every group was measured ventricle function and simultaneity weighted the heart after 2 weeks and 4 weeks.HE stain was used to detect the myocardial infarction areas.Results:(1)14 days after ISO ①the left ventricle function:in group A,the LVSP、LVEDP、±dp/dt were higher than B group(P0.05);②the weight of left ventricle:in group A,the weight of heart/avoirdupois of rat was less than B group(P0.05);③the myocardial infarction areas:in group A,the myocardial infarction areas/the left ventricle overall areas was less than B group(P0.05);(2)In group A and B,there were the same results for 28 days comparing with for 14 days after injecting ISO.(3)the results of comparing between subgroups ①In A group,the ventricle function in the 4 weeks subgroup was higher than in the 2 weeks subgroup(P0.05),the myocardial infarction areas in the 4 weeks subgroup were smaller than in the 2 weeks subgroup(P0.05).The remodification of ventricle was not significant.②In B group,there were not significance difference of ventricle function、myocardial infarction areas and remodification of the left ventricle between subgroups.There was not statistics significance difference of heart rate between groups.Conclusions:G-CSF can protect and rebirth the ischemiced cardiac muscle of acute myocardial infarction in the rat models and improve the ventricle function in rats.