Utilization of ultrasound in using SDS reverse micelle
WU Qiu-ying
Abstract
WU Qiu-ying
Abstract
Studies was used for extracting soybean protein by dodecanesulphonate(SDS)/isooctane(octanol)reverse micelles system with ultrasonic-assisted in this paper.The main factors of effect reverse micelles extraction results had been analyzed and discussed.Compared with those under no ultrasonic,received the extract time and increased the extraction rate.The effects of factors had been investigated Orthogonal Test Design.The best techniques of forward extraction were obtained.The results showed the optimal conditions of backward extraction protein were ultrasound power 270W,WO about 20,temperature 40℃,0.05 mol/L,extract time 30 min,pH 8.0,0.015g/mL adding soybean powder.And the protein extraction rate was about 82.08%.
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Studies was used for extracting soybean protein by dodecanesulphonate(SDS)/isooctane(octanol)reverse micelles system with ultrasonic-assisted in this paper.The main factors of effect reverse micelles extraction results had been analyzed and discussed.Compared with those under no ultrasonic,received the extract time and increased the extraction rate.The effects of factors had been investigated Orthogonal Test Design.The best techniques of forward extraction were obtained.The results showed the optimal conditions of backward extraction protein were ultrasound power 270W,WO about 20,temperature 40℃,0.05 mol/L,extract time 30 min,pH 8.0,0.015g/mL adding soybean powder.And the protein extraction rate was about 82.08%.
Key concepts: Micelle, Extraction (chemistry), Chromatography, Ultrasound, Ultrasonic sensor, Chemistry, Octanol, Protein purification