Induction of apoptosis in HL-60 cells by a new antisense oligodeoxynucleotide targeting for the bcl-2 coding sequences

Aim: To observe the effect of bcl-2 antisense oligodeoxynucleotide targeting the coding region of the bcl-2 mRNA on the expression of bcl-2 protein and apoptosis in HL-60 cells. Methods: Cell viability、the expression of bcl-2 protein and apototic cells were observed by propidium iodide exclusion and flow cytometric. Results: The bcl-2 antisense oligodeoxynucleotide of 10 μmol/L and 20 μmol/L can inhibit bcl-2 protein expression and promote apoptosis of HL-60 cells , and the antisense oligodeoxynucleotide targeting the coding region of the bcl-2 mRNA has stronger action than that targeting the transtration initiation. Conclusion: The bcl-2 antisense oligodeoxynucleotide targeting the coding region of the bcl-2 mRNA can inhibit bcl-2 protein expression and promote apoptosis of HL-60 cells.[