[Inhibitive effect of bcl-2 antisense oligodeoxynucleotide on Bcl-2 protein synthesis and cell proliferation in human laryngocarcinoma Hep-2 cells].

OBJECTIVES: To evaluate the role of bcl-2 antisense oligodeoxynucleotide in the gene treatment of human laryngocarcinoma. METHODS: An antisense oligodeoxynucleotide complementary to the initiation coden and the next four codens of bcl-2 mRNA was synthesized with an automatic DNA synthesizer. It was used to treat cultured Hep-2 cells to observe its effect on cell proliferation. In situ hybridization method and immunohistochemical technology were adopted to detect bcl-2 mRNA and protein and explore the effect of bcl-2 antisense oligodeoxynucleotide fragment on bcl-2 gene expression and protein synthesis and inhibition of cell proliferation. RESULTS: Bcl-2 antisense oligodeoxynucleotide had an obvious effect on bcl-2 mRNA, but inhibited Bcl-2 protein synthesis significantly, the inhibitive rate had positive correlation with concentration of antisense oligodeoxynucleotide and time of action. Twenty mumol/L of antisense oligodeoxynucleotide could inhibit cell proliferation effectively. CONCLUSIONS: It is suggested that bcl-2 antisense oligodeoxynucleotide might specifically inhibit bcl-2 protein synthesis and cell proliferation in Hep-2 cells at translation level.