Effect of bcl-2 antisense oligonucleotide targeting different region of bcl-2 mRNA on drug-sensitivity of leukemia cells

Aim To explore the effects of bcl_2 antisense oligonucleotide targeting the coding region of the bcl_2 mRNA on apoptosis induced by Vp16 in HL60 and K562 cells.Methods Drug sensitivity was compared by MTT cytotoxicity assay, and expression of bcl_2 protein and apototic cells were assayed by flow cytometry.Results The bcl_2 antisense oligonucleotide of 10 μmol·L-1 combined with etoposide inhibited expression of bcl_2 protein,increased apoptosis in HL60 and K562 cells and decreased IC50 of etoposide; The antisense oligonucleotide targeting the coding region of the bcl_2 mRNA had stronger effection than the antisense oligonucleotide targeting the translation initiation.Conclusion The bcl_2 antisense oligonucleotide targeting the coding region of the bcl_2 mRNA can enhances etoposide induced apoptosis of HL60 and K562 cells.