Resveratrol effects cell multiplication and cycle of Mouse embryonic fibroblast

AIM:To discuss the cell multiplication and cell cycle situation in mouse embryonic fibroblast(MEF),which acted by Resveratrol (Res). METHODS: Res with different concentration(0.15-500 μmol/L)was used on MEF. After 72 h,cell multiplication situation and Inhibitory rate of cell growth were tested by MTT,and cell cycle was analyzed by using flow cytometry. RESULTS: A of the group with 1 μmol/L Res was Significant higher than the control group (P0.05). A of groups with 50 μmol/L and 150 μmol/L were 0.196±0.055 and 0.185±0.034 separately,which were lower than the control group (P0.05,P0.01 separately),and the inhibitory rates of cell growth were 14.0% and 18.9% separately. Cell cycle analysis showed that the group with 1 μmol/L Res had (14.82±0.49)% cells in G2/M period,which was higher than the control group (P0.01). The 50 μmol/L and 150 μmol/L groups had (59.61±6.03)% and (65.31±0.27)% cells in G0/G1 period,those were higher than the control group(P0.01). CONCLUSION: Two-way effect,which relies on the dose of Res,is showed on MEF. Multiplication and growth inhibition had relation with the control of cell cycle. Prevent cell turn into S period from G1 period in cell cycle is main inhibition growth effect in MEF.