Effects of Shenqi Xiezhuo Decoction on MMP-9 and TIMP-1 Expression in Adenine-induced Renal Interstitial Fibrosis Rats

Objective: To investigate the effects of Shenqi Xiezhuo Decoction on matrix metalloproteinase-9( MMP-9) and tissue inhibitor of matrix metalloproteinase-1( TIMP-1) expression in adenine-induced renal interstitial fibrosis rats. Methods:The renal failure model of rats was established by Yokozawa method. All rats were random Ly divided into 5 groups: high-dose group,regular-dose group,losartan group,model group and blank group. After 8 weeks,all rats' left kidney and blood were analyzed by RT-PCR and serum biochemical tests. Results: 1The level of serum creatinine of high-dose group was significantly lower than the levels of regular-dose group or losartan group and there was no significant difference between the latter two groups. 2The expression of MMP-9 of high-dose group was more than that of the regular-dose group or losartan group and there was no significant difference between the latter two groups. The expression of TIMP-1 of high-dose group was less than that of the regular-dose group or losartan group and there was no significant difference between the latter two groups. 3There was no difference of the level of ALT between blank group and the others. Conclusion: Shenqi Xiezhuo Decoction may alleviate deterioration of the renal function by regulating MMP-9 and TIMP-1 balance.