The study of anti-inflammatory mechanisms of procyanidin on cerebral ischemia reperfusion injury of rats

Objective To investigate the Anti-Inflammatory mechanisms of grape seed procyanidin(GSP)on cerebral ischemia reperfusion injury(CIRI)of rats.Methods The model of MCAO was established by intraluminal suture technique.After 2 h of occlusion and 24 h of reperfusion,The high dose GSP(40 mg/kg)group and low dose GSP(10 mg/kg)group.was injected by intraperitoneal injection for 7 days,and all groups were given a more time in 15 minutes before operation.The contents of NO and activities of MPO in brain tissue of ischemic hemisphere were measured;the expression of ICAM-1 in the cortex were measured by immunohistochemical method.Results The neurological scores of rats treated with GSP was reduced apparently compared with model group rats(P0.05);there were apparent reduction of the contents of NO and marked decrease of MPO in GSP group compared with the I/R model group(P0.05);compared with I/R model group,the expression of ICAM-1 was down-regulated obviously in GSP group(P0.05).Conclusion GSP could relieve the cerebral ischemia reperfusion injury,one of the mechanisms could be the Anti-Inflammatory effect of procyanidin