Effect of Ruangan Huajian granule on hepatic expression of PDGF-B in C57 mice with experimental hepatic fibrosis

Objective To observe the effect of Ruangan Huajian granule on hepatic expression of PDGF-B in C57 mice with experimental hepatic fibrosis.Methods Fifty C57 mice were randomly divided into five groups(10 mice in each group),i.e.normal control group,control model group,low-dose Ruangan Huajian granule model group,high-dose Ruangan Huajian granule model group and colchicine model group.C57 mice with experimental hepatic fibrosis were induced by intraperitoneal injection of 10% CCl4.The expression of PDGF-B in liver tissue of mice was observed by immune histochemical technique.Compare the expression of PDGF-B in different Ruangan Huajian granule dosage group with other experimental groups.The scores of inflammation grade(G) and fibrosis stage(S) in liver tissue were also analyzed.Results The expression of PDGF-B and the scores of G and S in model groups were significantly higher than those in normal control group(P0.05),and that of model control group were significantly higher than Ruangan Huajian granule low-dose model group,Ruangan Huajian granule high-dose model group and colchicine model group(P0.05).There was no significant difference among Ruangan Huajian granule low-dose model group,Ruangan Huajian granule high-dose model group and colchicine model group(P0.05).Conclusion Ruangan Huajian granule may alleviate the degree of liver inflammation and fibrosis through reducing hepatic expression of PDGF-B in C57 mice with experimental hepatic fibrosis.