Extraction Technology of Chlorophyll from Urtica laetevirens Maxim Leaves

[Objective] To study the effects of different conditions on the extraction efficiency of chlorophyll from U.laetevirens,so as to provide references for the development and utilization of U.laetevirens leaves.[Method] Condition experiment and orthogonal test were adopted to determine extraction solvent,solid-liquid ratio,temperature,extraction time and optimize the extraction process,and the extraction efficiency was compared.[Result] The optimal conditions for the extraction by using 80% acetone were as follows: 1∶20 of solid-liquid ratio at 56 ℃for 7 h.Influential factors are ranked as: solid-liquid ratio extraction temperatureextraction time.Ultrasonic assisted extraction process has the obvious advantages of rapid and higher efficiency over traditional extraction method.[Conclusion] Solid-liquid ratio is the major factor influencing the extraction efficiency,and the ultrasonic assisted extraction process can greatly improve the extraction rate.