Extraction technique of total flavonoids from leaves of Azadirachta indica

To optimize extraction method and extraction technique of total flavonoids from neem leaves, effects of different conditions including extraction temperature, ethanol concentration and extraction time on extraction efficiency were studied by using single factor and orthogonal experimental design. Results showed that the optimum extraction conditions were extraction temperature of 70℃, 70% ethanol and extraction time of 30 min. The extraction process was stable. Thus, the optimum extraction technology is stable, the extraction method requires short time, low production cost and has high extraction efficiency.