A Study on Establishment of Gestational Diabetes Rat Model with Different Doses of Streptozotocin

Objective:To establish gestational diabetes (GD) rat model with different doses of streptozotocin(STZ),and to investigate the optimal dose. Methods:Female SD rats and male rats were caged together with the proportion of 1:2. The time when sperm cells were observed in female rats under microscope was recorded as pregnancy 0d. Fifty five pregnant rats were randomly divided into 3 groups. On the 6th day of pregnancy after fasting 12 h,GD groups were injected with 45 mg/kg (group D45) or 55mg/kg (group D55) of STZ once through abdominal cavity respectively and control group was injected with citrate buffer solution instead. Fasting blood glucose,urine glucose,success rate,mortality and negative conversion rate were observed. Results:Compared with those of control group,blood glucose levels and urine glucose levels of GD groups increased significantly. Success rates of groups D45 and D55 were 95.0% and 100.0% respectively; Death rates and negative conversion rates were 5.0%,33.3% and 10.52%,6.67% respectively. The only statistically significant difference between group D45 and group D55 was mortality. Conclusion:The ideal dose of STZ for establishment of GD rat model is about 45mg/kg by injection through abdominal cavity,which leads to higher success rate,lower death rate,and lower negative conversion rate.