Comparison on the stability of the mouse model of gestational diabetes established by different doses of streptozotocin

Objective To investigate the effect of different doses of streptozotocin(STZ) on the stability of the mouse model of gestational diabetes established by streptozotocin. Methods Forty C57BL/6J pregnant mice were randomly divided into the control group(10 mice) and the streptozotocin group(20 mg/kg STZ group, 50 mg/kg STZ group and 100 mg/kg STZ group, each with 10 mice). The mice were injected intraperitoneally with equal dose of citrate buffer, STZ solution 20 mg/kg, 50 mg/kg, 100 mg/kg, respectively, according to their assigned groups, andthen they were given normal feeding. The fasting blood glucose and body mass were measured on the third, 10th, 18th day of pregnancy, and the water intake and urine output changes were also observed. Finally the modeling rates of the pregnant mice were compared between the different groups. Results The modeling rate was found to be 40% for 20 mg/kg STZ group, 70% for 50 mg/kg STZ group, 50% for 100 mg/kg STZ group. Compared with the control group, the levels of fasting blood glucose was significantly higher in 50 mg/kg STZ group, and the body weight was significantly lower(P0.05). The state of hyperglycemia retained continually and steadily. Conclusion 50 mg/kg is the optimal dose for intraperitoneal injection of streptozotocin into C57BL/6J pregnant mice to establish gestational diabetes mouse model, with good stability.