Purification of Flavonoids from Mesosperms of Ginkgo biloba with Macro Porous Adsorbing Resins

In this paper,the extracts of the mesosperms of Ginkgo biloba were purified with three kinds of macro porous adsorbing resins.The adsorption rate,desorption rate and adsorption kinetics of each resin were studied,and some technical conditions were optimized via static and dynamic experiments.The results showed that weak polar AB-8 and DM-130 were good sorbents of flavonoids.In static experiments,the optimum adsorption conditions of the two resins were about the same:extracts pH 3,resin:extracts was 1:20,adsorption temperature 40 ℃ and eluant was 70% ethanol.The dynamic experiments suggested that flavonoids from mesosperms of Ginkgo biloba could be eluted from AB-8 and DM-130 with seven times and nine times of 70% ethanol respectively.The content of flavonoids in the purified extracts reached 16.3% with AB-8 in the optimized conditions.