Effect of Wendan Decoction on SOD,MDA,NO and PKC in Rats Serum with Schizophrenia

Objective: To research the effect of Wendan decoction [It was made up of Pinelliae Rhizoma,Poria,Bambusae Caulis in Taenias,Aurantii Fructus Immaturus,Citri Reticulatae Pericarpium,Glycyrrhizae Radix et Rhizoma Prasparata Cum Melle.] on superoxide dismutase(SOD),malondialdehyde(MDA),nitric oxide(NO) and protein kinase(PKC) in rats serum with schizophrenia.Method: Fifty SD rats were randomly divided five groups: normal group(A),model group(B),Wendan decoction high-dosage group(C),Wendan decoction medium-dosage group(D) and Wendan decoction low-dosage group(E),ten rats in each group.Before established the model,the C,D,E groups were given Wendan decoction(40,20,10 g.kg-1) and the A and B groups were given normal saline every day,which last for 21 days.After two hours at the last given Wendan decoction or saline,the B,C,D,E groups were ip given MK801 0.6 mg.kg-1 to establish rat schizophrenia the model at the left side of the rat abdominal cavity.After general condition of rats was observed three days,the rats were sacrificed and serum was tested.The superoxide dismutase(SOD),malondialdehyde(MDA),nitri oxide(NO) in rats serum were detected by biochemical methods and the PKC in rats serum was detected by double antibody clip ELASA method.Result: Compared with B group,general condition of rats was improved,the MDA,NO,PKC content of group C,D and E were significantly decreased(P 0.05 or P 0.01) and their SOD was significantly increased(P 0.05 or P 0.01),pathological damage caused by oxygen free radical was alleviated.The PKC content of group C,D and E were significantly decreased(P 0.05 or P 0.01).Conclusion: Wendan decoction could reduce the pathologic lesions of rat with schizophrenia,protect from the damage caused by oxygen free radicals;Wendan decoction could decrease PKC content and thus play a regulatory role in the function of GJIC.