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MUTANT STRAIN SCREENING AND ITS ENZYME PRODUCTION CONDITIONS OF CELLULASE

Dong Zhi, Min Lin

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Abstract

Trichoderma kningii T\|801,which can produce relatively high cellulase,was isolated.The ability of producing cellulase of mutant T\|801 had increased 1 77 times after treated with nitrousguanide and γ ray and was higher than that of Trichoderma QM9414 The medium with straw powder as carbon source and peptone as nitrogen source is optimal and the maximum cellulase activity is reached at 30℃ and pH5 0 when cultured for 5days.

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What this paper is about

Trichoderma kningii T\|801,which can produce relatively high cellulase,was isolated.The ability of producing cellulase of mutant T\|801 had increased 1 77 times after treated with nitrousguanide and γ ray and was higher than that of Trichoderma QM9414 The medium with straw powder as carbon source and peptone as nitrogen source is optimal and the maximum cellulase activity is reached at 30℃ and pH5 0 when cultured for 5days.

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Available abstract

Trichoderma kningii T\|801,which can produce relatively high cellulase,was isolated.The ability of producing cellulase of mutant T\|801 had increased 1 77 times after treated with nitrousguanide and γ ray and was higher than that of Trichoderma QM9414 The medium with straw powder as carbon source and peptone as nitrogen source is optimal and the maximum cellulase activity is reached at 30℃ and pH5 0 when cultured for 5days.

Key concepts: Cellulase, Carbon source, Strain (injury), Trichoderma, Straw, Mutant, Chemistry, Enzyme

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