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Study on Optimization Producing Cellulase Conditions of Trichoderma

Lin Ying

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Abstract

In this paper,the producing cellulase conditions of mutant stain Trichoderma F-UV_(264) were stduied.The result of experiment showed that: the optimal nitrogen source is 1%(NH_4)_2SO_4 and 3%the surface active material,the optimal sult group is 2%Mandels,bran:straw is 1∶3,solid:liquid is(1∶4).The enzyme vitality of cellulase can be increased after adding these influental factors.The optimum culture condition of producing cellulase is pH(4.6)～5.6 at 30 ℃ for 96～135 h,and the maximum cellulase activity is reached.This research can provide the basis for the cellulase manufacture.

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What this paper is about

In this paper,the producing cellulase conditions of mutant stain Trichoderma F-UV_(264) were stduied.The result of experiment showed that: the optimal nitrogen source is 1%(NH_4)_2SO_4 and 3%the surface active material,the optimal sult group is 2%Mandels,bran:straw is 1∶3,solid:liquid is(1∶4).The enzyme vitality of cellulase can be increased after adding these influental factors.The optimum culture condition of producing cellulase is pH(4.6)～5.6 at 30 ℃ for 96～135 h,and the maximum cellulase activity is reached.This research can provide the basis for the cellulase manufacture.

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Available abstract

In this paper,the producing cellulase conditions of mutant stain Trichoderma F-UV_(264) were stduied.The result of experiment showed that: the optimal nitrogen source is 1%(NH_4)_2SO_4 and 3%the surface active material,the optimal sult group is 2%Mandels,bran:straw is 1∶3,solid:liquid is(1∶4).The enzyme vitality of cellulase can be increased after adding these influental factors.The optimum culture condition of producing cellulase is pH(4.6)～5.6 at 30 ℃ for 96～135 h,and the maximum cellulase activity is reached.This research can provide the basis for the cellulase manufacture.

Key concepts: Cellulase, Bran, Trichoderma, Chemistry, Straw, Food science, Enzyme, Pulp and paper industry

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