Effects of Vernoniaanthelmintica Willd on the immune function in normal mice

Objective To explore the effect of the Vernoniaanthelmintica willd on the immune function of mice. Methods The effect of Vernoniaanthelmintica willd on the spleen T lymphocyte proliferation stimulated by Con A, B lymphocyte proliferation stimulated by LPS and the spleen T lymphocyte secretion of IL 2 activity were assessed by TdR incorporation respectively, antibody production in the serum were assessed by ELISA,spleen B lymphocyte surface antigen (CD19) of mice were assessed by flow cytometry.Results Vernoniaanthelmintica willd inhibited spleen T, B lymphocyte proliferation and T lymphocyte secretion of IL 2 activity, the total antibody and specific antibody production to human A375 melanoma cell and surface antigen (CD19) of B lymphocyte expression on normal mouse in vivo .Conclusion Vernoniaanthelmintica willd could inhibit the humoral and cellular immunity function on normal mouse.