Effect of Polysaccharides from Ganoderma atrum on Spleen Lymphocyte Proliferation and Induction of Cytokine in Mice

Objective:To investigate the effect of polysaccharides from Ganoderma atrum (PSG-1) on spleen lymphocyte proliferation and induction of cytokine in mice. Methods:The effects of treatments with PSG-1 and with PSG-1 containing motigen at different concentrations on spleen lymphocyte proliferation were determined by MTT. The contents of IL-2 and TNF-α were determined by using ELISA. Results:PSG-1 at a concentration ranging from 20 to160 μg/mL resulted in a significant increase of lymphocyte proliferation,and 48 h administration gave the best proliferation. PSG-1 could also enhance the effect of Con A-induced T lymphocyte proliferation and the stimulation index of LPS-induced B lymphocytes. Moreover,PSG-1 could significantly increase the secretion of IL-2 and TNF-α in spleen lymphocyte. Conclusion:PSG-1 can effectively increase lymphocyte proliferation and enhance the effect of Con A,LPS-induced T and B cell growth. PSG-1 has the ability to enhance immune responses by increasing the secretion of IL-2 and TNF-α.