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Determination of nitrofuran metabolites in freshwater by ultra performance liquid chromatography-tandem mass spectrometry

Le Yuan

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Abstract

An ultra performance liquid chromatography-tandem mass spectrometric(UPLC/MS/MS) method was established for determining 4 nitrofuran metabolites in freshwater,namely 1-aminohydantoin hydrochloride(AHD),3-amino-5-morpholinomethyl-2-oxazolidineone(AMOZ),3-amino-2-oxazolidinone(AOZ) and semicarbazide(SEM).The samples were subjected with dilute hydrochloric acid then derivatized with 2-nitrobenzene formaldehyde.The analysis was carried out in the multi reaction monitoring(MRM) mode and internal standard isotope dilution method was used for quantification.Calibration curves exhibited good linearity over the concentration range from 1.0 to 100 ng/mL and their limits of detection were all 0.5 μg/L.Average recoveries for them spiked at 4 levels ranged from 75.1% to 102%,with a RSD of less than 15%.

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What this paper is about

An ultra performance liquid chromatography-tandem mass spectrometric(UPLC/MS/MS) method was established for determining 4 nitrofuran metabolites in freshwater,namely 1-aminohydantoin hydrochloride(AHD),3-amino-5-morpholinomethyl-2-oxazolidineone(AMOZ),3-amino-2-oxazolidinone(AOZ) and semicarbazide(SEM).The samples were subjected with dilute hydrochloric acid then derivatized with 2-nitrobenzene formaldehyde.The analysis was carried out in the multi reaction monitoring(MRM) mode and internal standard isotope dilution method was used for quantification.Calibration curves exhibited good linearity over the concentration range from 1.0 to 100 ng/mL and their limits of detection were all 0.5 μg/L.Average recoveries for them spiked at 4 levels ranged from 75.1% to 102%,with a RSD of less than 15%.

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Available abstract

An ultra performance liquid chromatography-tandem mass spectrometric(UPLC/MS/MS) method was established for determining 4 nitrofuran metabolites in freshwater,namely 1-aminohydantoin hydrochloride(AHD),3-amino-5-morpholinomethyl-2-oxazolidineone(AMOZ),3-amino-2-oxazolidinone(AOZ) and semicarbazide(SEM).The samples were subjected with dilute hydrochloric acid then derivatized with 2-nitrobenzene formaldehyde.The analysis was carried out in the multi reaction monitoring(MRM) mode and internal standard isotope dilution method was used for quantification.Calibration curves exhibited good linearity over the concentration range from 1.0 to 100 ng/mL and their limits of detection were all 0.5 μg/L.Average recoveries for them spiked at 4 levels ranged from 75.1% to 102%,with a RSD of less than 15%.

Key concepts: Chromatography, Chemistry, Nitrofuran, Semicarbazide, Detection limit, Tandem mass spectrometry, Mass spectrometry, Nitrobenzene

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