Determination of Metabolites of Nitrofuran Antibiotics in Pre-export Pork by High Performance Liquid Chromatography-Tandem Mass Spectrometry

In this experiment, high performance liquid chromatography-tandem mass spectrometry(LC-MS-MS) was used to detect four nitrofuran metabolites in per-export pork , namely 3-amino-2-oxazolidinone(AOZ), 3-amino-5-morpholinomethyl- 2-oxazolidineone (AMOZ), 1-aminohydantoin hydrochloride(AHD) and semicarbazide(SEM). Samples were dericatized with 2-nitrobenzaldehyde at pH 7.0 to 7.5 , the derivatized solutions were cleaned up by Oasis HLB solid extraction column (SPE), and the target compounds were eluted with carbinol and diluted with mobile phase. MS detection was performed in the positive ion mode using multiple reaction monitoring. Results showed that the recoveries of these four metabolites range from 98.95% to 99.98% , their limits of detection are 0.5 mg/kg, and their relative standard devitions are not more than 4.22% (n=10).