Rapid determination of four nitrofuran metabolites in honey by isotope dilution-tandem mass spectrometry

A method for the determination of four nitrofuran metabolites such as 3-amino2-oxazolidinone(AOZ),5-morpholinomethyl-3-amino-2-oxazolidinone(AMOZ),1-aminohydantoin(AHD),and semicarbazide in honey has been established by isotope dilution high performance liquid chromatography-tandem mass spectrometry.Samples were derived with 2-nitrobenzaldehyde and(AOZ-d4) d4-3-amino-2-oxazolidinone,and d5-5-morpholinomethyl-3-amino-2-oxazolidinone(AMOZ-d5),13C isotope of 1-aminohydantoin(AHD-13C3),13C and 15N isotope of semicarbazide(SEM·HCl-13C15N2) were used as internal standards.Extracted with ultrasonic,the analytes were separated and analysed by gradient elution of acetonitrile and 0.1% formic acid in 15 min.The average recoveries were 82%～112%;limits of quantification(LOQ) of 0.05～1 μg/kg and limits of detection(LOD) of 0.01～0.25 μg/kg were achieved using tandem mass spectrometry.All of the analytical results satisfied the detection demands of European Union for nitrofuran metabolites in import export honey.