Preparation of solid lipid nanoparticles containing praziquantel

Objective To prepare solid lipid nanoparticles containing praziquantel(PZQ-SLN) by ultrasonic technique,and investigate the main factors in the process of preparing PZQ-SLN.Methods At first the parameters of technology were determined by experiments,then the affecting factors of the size and stability of PZQ-SLN were researched.On the basis of the single factor exploration,the satisfactory formulation was selected by orthogonal design with high entrapment efficiency as standard.Results The morphological investigation by transmission electron microscopy showed that the nanoparticles had round and uniform shapes.The mean diameters was(100±21)nm,accordingly the drug entrapment efficiencies was(79.3±0.69)%,and the zeta potential was-66.3 mV.Conclusions The solid lipid nanoparticles loaded with praziquantel can be readily and quickly prepared by ultrasonic technique,with Compritol 888 ATO and butyl acetate as matrix material,soybean lecithin,poloxamer 188 and sodium stearate as co-emulsifier.