Preparation of Rutin Solid Lipid Nanoparticles by film-ultrasonic method

Objective To optimize the technique for preparing Rutin Solid Lipid Nanoparticles(RT-SLN)by film-ultrasonic dispersion.Methods The formulation was optimized by an orthogonal design.The entrapment efficiency was used as an index to investigate the factors of stearic acid,leci-thin,Tween-80 concentration and PEG-400 concentration.The electron microscope was used to observe the surface of RT-SLN by negative-dying.Particle size and Zeta potential were determined by laser particle analyzer,and drug release in vitro was evaluated by dialysis.Results RT-SLN were spherical.Its partical size was 195.8±11nm,Zeta potential was-20.65±0.6mV,and the entrapment efficiency was 86.31%.The cumulative release rate was 87.32% within 72 hours in vitro.Conclusion Rutin Solid Lipid Nanoparticals could be prepared under the optimized conditions with high entrapment efficiency and in vitro sustained release characteristics.