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2006•Chinese Journal of Spectroscopy LaboratoryRequires access

Determination of Rutin and Quercetin in Limonium Bicolor Stem by HPLC

An Hong

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Abstract

The contents of rutin and quercetin in Limonium bicolor were determined by HPLC with a Hypersil ODS2 column(4.6mm ×250mm,5μm)at room temperature,CH_3OH-0.5% H_3PO_4 solution as mobile phase at a flow of 1mL/min and UV detection at 355nm.The linear range is 0.12—1.20μg for rutin(r=0.9999) and 0.04—0.40μg for quercetin(r=0.9997).The recovery are 101.42% (RSD=0.98%) for rutin and 102.85%(RSD=1.32%) for quercetin.The method was simple,and had good linear relationship.

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What this paper is about

The contents of rutin and quercetin in Limonium bicolor were determined by HPLC with a Hypersil ODS2 column(4.6mm ×250mm,5μm)at room temperature,CH_3OH-0.5% H_3PO_4 solution as mobile phase at a flow of 1mL/min and UV detection at 355nm.The linear range is 0.12—1.20μg for rutin(r=0.9999) and 0.04—0.40μg for quercetin(r=0.9997).The recovery are 101.42% (RSD=0.98%) for rutin and 102.85%(RSD=1.32%) for quercetin.The method was simple,and had good linear relationship.

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Available abstract

The contents of rutin and quercetin in Limonium bicolor were determined by HPLC with a Hypersil ODS2 column(4.6mm ×250mm,5μm)at room temperature,CH_3OH-0.5% H_3PO_4 solution as mobile phase at a flow of 1mL/min and UV detection at 355nm.The linear range is 0.12—1.20μg for rutin(r=0.9999) and 0.04—0.40μg for quercetin(r=0.9997).The recovery are 101.42% (RSD=0.98%) for rutin and 102.85%(RSD=1.32%) for quercetin.The method was simple,and had good linear relationship.

Key concepts: Rutin, Quercetin, Chemistry, Chromatography, High-performance liquid chromatography, Linear range, Linear relationship, Detection limit

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