Study on Expressions and Clinical Significance of PTEN,p-Akt1 by Immunohistochemical Staining and Tissue Microarray in Human Colon Cancer

Objective To study the expressions and clinical significance of PTEN,p-Akt1 in colon cancer,adenomatous polyps and corresponding normal para-cancer colon mucosa.Methods Eighty-five cases of colon cancer,18 cases of adenomas and 9 cases of para-cancer mucosa were accumulated and two tissue microarrays were made which containing 72 dots and 104 dots respectively.Expressions of PTEN,p-Akt1 proteins were detected by immunohistochemical staining to these tissue microarrays.Re-sults No significant difference was found among varied tissues according to the expression of PTEN and p-Akt1(P0.05).In addition,expression of PTEN has no correlation with other clinicopathological parameters but histological grades(P0.05).No correlations of expression of p-Akt1 with clinicopatholog-ical data but histological grade(P0.001) and lymph node metastasis(P0.05) were found.Conclusion Though not involving in the development of colon carcinigenesis,distortion of PTEN and p-Akt1 may have potent roles in progression of colon cancer.