2009•Shenyang Yaoke Daxue xuebaoRequires access
Determination of content of protocatechuic acid from the seeds of Castanea mollissima Blume by HPLC
WU Li-jun
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Abstract
Objective To set up an HPLC method for the determination of the contents of protocatechuic acid in pre-and post-acid hydrolyzed extracts from the seeds of Castanea mollissima Blume.Methods HPLC operating conditions were Diamonsil C18(200 mm×4.6 mm,5 μm)at room temperature,methanol-water-phosphoric acid(V∶V∶V=8.0∶92.0∶0.1)as the mobile phase at a flow rate of 0.8 mL·min-1 and UV detection at 259 nm.Results Contents of protocatechuic acid in pre-and post-acid hydrolyzed were 8.12×10-3 %(w) and 1.82×10-2 %(w),respectively.Conclusions The method is suitable for the determination of protocatechuic acid in the seeds of Castanea mollissima Blume.After acid hydrolyzation,the contents of protocatechuic acid in C.mollissima Blume increased evidently.