Preparation of Highly Efficient Degradation Strain Immobilized Cells for Phenol and Its Degradation Conditions

The strain which can use phenol as only carbon source,was isolated by domestication of sludge from a printing plant,the highly efficient degradation strain was immobilized in the sodium alginate.The optimal preparation condition of the strain immobilized cells was determined by orthogonal test.The results show that the degradation efficiency of immobilized cells is apparently superior to that of free cells,and the optimal temperature of degrading phenol is 30～35 ℃,the optimal pH value is 7～9,the rotation speed is 120～150 r /min,1 200 mg/L phenol can be completely degraded after 30 h reaction.