Immobilization and Degradation Perpormance of Efficient Phenol-degrading Bacteria Bacillus sp.JY01

One strain of phenol-degrading Bacillus sp.JY01 was immobilized in sodium alginate.Optimal preparation condition of the strain immobilization was ascertained by the cross-test experiments.The phenol-degrading performance of the immobilized cells and free cells were studied and compared.Results indicated that sodium alginate 's mass fraction was 3% and the volume ratio for bacterial:sodium alginate aqueous solution was 4:30,CaCl2 content was 3%,calsifying time was 8h and the best temperature of degrading phenol was 32℃ ,the optimal pH value range was 7.0 ～7.5.Under this condition,immobilized microorganism can degradate phenol solution with concentration of 1300mg/L efficiently.After immobilized cells were recycled 8 times phenol degradation rate still reached 96.8% .Results showed that the degradation performance of immobilized cells was apparently superior to that of free cells.This experiment offered a comparatively reliable parameter for the phenolic effluent biological treatment by this bacterial.