Study on Immobilization of Phenol-Degrading Strain and Its Degradation Performance

In this paper,the phenol-degrading strain was immobilized on sodium alginate.The optimal immobilization conditions were determined by single factor experiment as follows: sodium alginate of 3.0%,CaCl2 of 4.0%,amount of wet strain of 0.4 g.The optimum conditions for immobilized cell degrading phenol were as follows:temperature of 30℃,pH value of 7.0,NaCl mass concentration lower than 2.5%.The results showed that the degradation performance and tolerance of immobilized cells for phenol were apparently superoir to those of free cells.When 800 mg·L-1 of phenol was degraded by immobilized cell for 48 h,its degradation rate reached over 99%.