Relationship among expression of Cyclin D_1、p16、p27 and PCNA in glioma

Objective:To investigate the expression of Cyclin D 1?p16?p27 and PCNA in glioma with different pathological classification, and to explore the correlationship among Cyclin D 1?p16?p27 and PCNA.Methods:Cyclin D 1?p16?p27 and PCNA protein expressions in 12 normal brain tissues and 58 glioma cases were examined by immunohistochemical staining and hemiquantity analysis.Results:With the increasing of glioma malignancy, the expression level of Cyclin D 1 protein increased gradually (P0.005, χ 2=51.67),while the expression lever of p16?p27 decreased (P0.005 both, χ 2 is for 15.41 and 12.81,respectively).Positive correlation was found between Cyclin D 1 and PCNA (rs=0.745).There was negative correlation between p16?p27 and PCNA rs parting for -0.566 and -0.612.Conclusion:In the process of glioma cell proliferation,Cyclin D 1 may be facilitate, while p16 and p27 may be inhibitory.