Interfering effect of ligustrazine on lipid peroxidation during renal ischemia-reperfusion injury in rabbits

Objective To observe the change of lipid peroxidation during renal ischemia-reperfusion injury, and to investigate the effect of ligustrazine on it. Methods 30 rabbits were divided into 3 groups randomly (each n = 10): control group, ischemia-reperfusion group (IR group), and ligustrazine group (LZ group). The blood was gathered from common carotid artery at pre-ischemia, ischemia 1h, reperfusion 5 h to detect the activity of xanthine oxidase (XO) and superoxide dismutase (SOD), and the content of malondialdehyde (MDA). The renal tissue was collected at the end of experiment to detect the activity of XO and SOD and the content of MDA, and to observe the ultrastructural changes with electronic microscope. Results The activity of XO and the content of MDA in plasma and renal tissue were significant lower, while the activity of SOD in plasma and renal tissue were significant higher in LZ group than those in IR group (P 0.01 or P 0.05). There were serious ultrastructural changes in glomerular capillary endothelium and podocyte / capsule epithelium in IR group, but were alleviated in LZ group. Conclusion Ligustrazine may decrease the generation and enhance the clearance of oxygen free radical, and restrain lipid peroxidation, and finally attenuate renal ischemia-reperfusion injury.